Identification of Self-Incompatibility Genotypes in Some Sand Pears (Pyrus pyrifolia Nakai) by PCR-RFLP Analysis

The identification of self-incompatibility genotype (S-genotype) will be useful for selection of pollinizers and design of crossing in cultivar improvement of sand pear. This paper reported the identification of self-incompatibility genotypes of seven Chinese and two Japanese sand pear cultivars using PCR-RFLP analysis and S-Rnase sequencing. The S-genotypes of these cultivars were determined as follows: Huali 1 S1S3, Shounan S1S3, Xizili S1S4, Qingxiang S3S7, Sanhua S2S7, Huangmi (Imamuranatsu) S1S6, Huali 2 S3S4, Baozhuli S7S33, Cangxixueli S5S15. S-Rnase alleles (S1 to S9) in sand pear could be identified effectively by PCR-RFLP analysis.

作 者： GU Qing-qing ZHANG Qing-lin HU Hong-ju CHEN Qi-liang LUO Zheng-rong   作者單位： GU Qing-qing(Key Laboratory for Horticultural Plant Biology of Ministry of Education (MOE)/Huazhong Agricultural University, Wuhan 430070, P.R. China;Department of Horticulture, Jiangxi Agricultural University, Nanchang 330045, P.R.China)

ZHANG Qing-lin,LUO Zheng-rong(Key Laboratory for Horticultural Plant Biology of Ministry of Education (MOE)/Huazhong Agricultural University, Wuhan 430070, P.R. China)

HU Hong-ju,CHEN Qi-liang(Fruit-Tree and Tea Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430209, P.R.China) 

刊 名： 中國農業(yè)科學（英文版）  英文刊名： AGRICULTURAL SCIENCES IN CHINA  年，卷(期)： 2009 8(2)  分類號： S1  關鍵詞： Pyrus pyrifolia Nakai   self-incompatibility genotype   S-allele   S-Rnase   PCR-RFLP   crossing experiment  

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